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Image Search Results
Journal: Neural Regeneration Research
Article Title: Chlorogenic acid alleviates hypoxic-ischemic brain injury in neonatal mice
doi: 10.4103/1673-5374.350203
Figure Lengend Snippet: CGA regulates the relative expression level of ferroptosis proteins after HIBD in newborn mice. (A–F) Western blot of SLC7A11, FHC, FLC, SLC3A2, GLS2, and GSS expression in the cortex ( n = 4). β-Actin was used as an internal reference. (G) Cox-2 , Nrf2 , GSH and GPX4 mRNA levels in hippocampus tissue 24 hours after HIBD; GAPDH mRNA was used as a normalization control ( n = 3). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, vs . Sham + NS group; # P < 0.05, ## P < 0.01, vs . HI + NS group (one-way analysis of variance followed by Tukey’s post hoc test). CGA: Chlorogenic acid; COX2: cyclooxygenase-2; FHC: ferritin heavy chain; FLC: ferritin light chain; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GLS2: glutaminase 2; GSH: L-glutathione; GSS: glutathione synthase; HI or HIBD: hypoxic-ischemic brain injury; Nrf2: nuclear factor (erythroid-derived 2)-like 2; NS: normal saline; SLC3A2: solute carrier family 3 member 2; SLC7A11: solute carrier family 7 member 11.
Article Snippet: The primary antibodies used included rabbit anti-4-hydroxynonenal (4-HNE; 1:2000, Abcam, Cat#ab46545, RRID: AB_722490), rabbit anti-glutathione peroxidase 4 (GPX4; 1:100, ABclonal, Wuhan, China, Cat# a1933, RRID:AB_2763960),
Techniques: Expressing, Western Blot, Derivative Assay
Journal: Neural Regeneration Research
Article Title: Chlorogenic acid alleviates hypoxic-ischemic brain injury in neonatal mice
doi: 10.4103/1673-5374.350203
Figure Lengend Snippet: CGA exerts neuroprotection via the system Xc – /GPX4 axis. (A–D) Western blot analysis of SLC3A2, SLC7A11, GSS, and GPX4 in the cortex. β-Actin was used as an internal reference. (E–H) Quantitative analyses of SLC3A2, SLC7A11, GSS, and GPX4 expression normalized with β-actin in the cortex. (I) The MDA level in the cortex. Data are presented as mean ± SEM ( n = 4). * P < 0.05, ** P < 0.01 (one-way analysis of variance followed by Tukey’s post hoc test). CGA: Chlorogenic acid; GPX4: glutathione peroxidase 4; GSS: glutathione synthase; HI or HIBD: hypoxic-ischemic brain injury; MDA: malondialdehyde; SLC3A2: solute carrier family 3 member 2; SLC7A11: solute carrier family 7 member 11.
Article Snippet: The primary antibodies used included rabbit anti-4-hydroxynonenal (4-HNE; 1:2000, Abcam, Cat#ab46545, RRID: AB_722490), rabbit anti-glutathione peroxidase 4 (GPX4; 1:100, ABclonal, Wuhan, China, Cat# a1933, RRID:AB_2763960),
Techniques: Western Blot, Expressing
Journal: Neural Regeneration Research
Article Title: Chlorogenic acid alleviates hypoxic-ischemic brain injury in neonatal mice
doi: 10.4103/1673-5374.350203
Figure Lengend Snippet: Schematic model for the mechanism of CGA in HIE. CGA inhibits ferroptosis by activating the system Xc – /GPX4 axis after HI. CGA: Chlorogenic acid; GCL: glutamate cysteine ligase; GLS: glutaminase; GPX4: glutathione peroxidase 4; GSH: L-glutathione; GSS: glutathione synthase; GSSG: L-glutathione oxidized; HIE: hypoxic-ischemic encephalopathy; ROS: reactive oxygen species; SLC3A2: solute carrier family 3 member 2; SLC7A11: solute carrier family 7 member 11; Xc – : the glutamate/cystine antiporter.
Article Snippet: The primary antibodies used included rabbit anti-4-hydroxynonenal (4-HNE; 1:2000, Abcam, Cat#ab46545, RRID: AB_722490), rabbit anti-glutathione peroxidase 4 (GPX4; 1:100, ABclonal, Wuhan, China, Cat# a1933, RRID:AB_2763960),
Techniques: